Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology
JD Van Embden, MD Cave, JT Crawford… - Journal of clinical …, 1993 - Am Soc Microbiol
JD Van Embden, MD Cave, JT Crawford, JW Dale, KD Eisenach, B Gicquel, P Hermans…
Journal of clinical microbiology, 1993•Am Soc MicrobiolDNA fingerprinting of Mycobacterium tuberculosis has been shown to be a powerful
epidemiologic tool. We propose a standardized technique which exploits variability in both
the number and genomic position of IS6110 to generate strain-specific patterns. General use
of this technique will permit comparison of results between different laboratories. Such
comparisons will facilitate investigations into the international transmission of tuberculosis
and may identify specific strains with unique properties such as high infectivity, virulence, or …
epidemiologic tool. We propose a standardized technique which exploits variability in both
the number and genomic position of IS6110 to generate strain-specific patterns. General use
of this technique will permit comparison of results between different laboratories. Such
comparisons will facilitate investigations into the international transmission of tuberculosis
and may identify specific strains with unique properties such as high infectivity, virulence, or …
DNA fingerprinting of Mycobacterium tuberculosis has been shown to be a powerful epidemiologic tool. We propose a standardized technique which exploits variability in both the number and genomic position of IS6110 to generate strain-specific patterns. General use of this technique will permit comparison of results between different laboratories. Such comparisons will facilitate investigations into the international transmission of tuberculosis and may identify specific strains with unique properties such as high infectivity, virulence, or drug resistance.
American Society for Microbiology