[HTML][HTML] Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin: genetic and biochemical evidence for complex formation of the LCB1 protein with the …
K Hanada, T Hara, M Fukasawa, A Yamaji… - Journal of Biological …, 1998 - ASBMB
Lysenin, a hemolytic protein derived from the earthworm Eisenia foetida, has a high affinity
for sphingomyelin. Chinese hamster ovary (CHO) cells exhibited a high cytolytic sensitivity to
lysenin, but treatment with sphingomyelinase rendered the cells resistant to lysenin.
Temperature-sensitive CHO mutant cells defective in sphingolipid synthesis were resistant
to lysenin, and this lysenin resistance was suppressed by metabolic complementation of
sphingolipids. Selection of lysenin-resistant variants from mutagenized CHO cells yielded …
for sphingomyelin. Chinese hamster ovary (CHO) cells exhibited a high cytolytic sensitivity to
lysenin, but treatment with sphingomyelinase rendered the cells resistant to lysenin.
Temperature-sensitive CHO mutant cells defective in sphingolipid synthesis were resistant
to lysenin, and this lysenin resistance was suppressed by metabolic complementation of
sphingolipids. Selection of lysenin-resistant variants from mutagenized CHO cells yielded …
