Purpose: To develop a molecular strategy of increasing cytotoxicity of chronically hypoxic
cells by inhibiting Glucose Regulated Protein 78 kDal (GRP78) induction. Methods and
Materials: A mutant nonGRP78 inducing cell line (78WO) was developed from its parent
(DG44) by overexpressing antisense GRP78 mRNA. Following exposure to varying
durations of hypoxia, Northern and Western blot analysis were used to characterize the
amount of GRP78 expression both at the RNA and protein level. Hypoxia was achieved by …