Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease
JR Bill, BL Kotzin - Arthritis Research & Therapy, 2002 - Springer
JR Bill, BL Kotzin
Arthritis Research & Therapy, 2002•SpringerMost techniques that identify antigen-specific T cells are dependent on the response of
these cells to the relevant antigen in culture. Soluble multimers of MHC molecules, when
occupied with the same peptide, will bind selectively to T cells specific for that MHC/peptide
complex. Techniques to produce fluorescent MHC class II/peptide multimers have recently
been developed. These reagents provide a method to facilitate detection and isolation of
antigen-specific CD4+ T cells and they represent a new research tool to study these cells in …
these cells to the relevant antigen in culture. Soluble multimers of MHC molecules, when
occupied with the same peptide, will bind selectively to T cells specific for that MHC/peptide
complex. Techniques to produce fluorescent MHC class II/peptide multimers have recently
been developed. These reagents provide a method to facilitate detection and isolation of
antigen-specific CD4+ T cells and they represent a new research tool to study these cells in …
Abstract
Most techniques that identify antigen-specific T cells are dependent on the response of these cells to the relevant antigen in culture. Soluble multimers of MHC molecules, when occupied with the same peptide, will bind selectively to T cells specific for that MHC/peptide complex. Techniques to produce fluorescent MHC class II/peptide multimers have recently been developed. These reagents provide a method to facilitate detection and isolation of antigen-specific CD4+ T cells and they represent a new research tool to study these cells in patients with immune-mediated diseases.
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