GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice
F Ahmed, J Wyckoff, EY Lin, W Wang, Y Wang… - Cancer research, 2002 - AACR
F Ahmed, J Wyckoff, EY Lin, W Wang, Y Wang, L Hennighausen, J Miyazaki, J Jones…
Cancer research, 2002•AACRTo examine the behavior of tumor cells in tumors developing directly from mammary tissue
in transgenic models, we have evaluated transgenic mice expressing green fluorescent
protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive
expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of
transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable
CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific …
in transgenic models, we have evaluated transgenic mice expressing green fluorescent
protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive
expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of
transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable
CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific …
Abstract
To examine the behavior of tumor cells in tumors developing directly from mammary tissue in transgenic models, we have evaluated transgenic mice expressing green fluorescent protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific. These animals provide a range of expression of GFP that is suitable for analysis of transgenic mammary tumors and metastases in vivo at the single cell level of resolution.
AACR
