Identification of the mitochondrial branched chain aminotransferase as a branched chain alpha-keto acid transport protein.
SM Hutson, TR Hall - Journal of Biological Chemistry, 1993 - ASBMB
Conditions were developed which optimized reconstitution of branched chain alpha-keto
acid transport activity, which was measured as alpha-ketoisocaproate (KIC) transport, and
pyruvate transport activity. Reconstitutable KIC transport activity was about 40-fold higher in
heart than in liver mitochondrial extracts and 40-fold higher than heart pyruvate transport
activity but only about 7-fold higher than liver pyruvate transport activity. A purification
procedure was developed for the branched chain alpha-keto acid and pyruvate transport …
acid transport activity, which was measured as alpha-ketoisocaproate (KIC) transport, and
pyruvate transport activity. Reconstitutable KIC transport activity was about 40-fold higher in
heart than in liver mitochondrial extracts and 40-fold higher than heart pyruvate transport
activity but only about 7-fold higher than liver pyruvate transport activity. A purification
procedure was developed for the branched chain alpha-keto acid and pyruvate transport …