3-Aminopropyltriethoxysilane (APES): a new advance in section adhesion.
PH Maddox, D Jenkins - Journal of clinical pathology, 1987 - ncbi.nlm.nih.gov
PH Maddox, D Jenkins
Journal of clinical pathology, 1987•ncbi.nlm.nih.govDiscussion An alcoholic solution of APES fulfils the requirements for an adhesive for the
immunostaining of pre-fixed frozen sections. It also provides a useful method of stabilising
histological and cytological prepara-tions on glass slides for other purposes. It is a
sufficiently simple, low cost technique (@ 50 pence for 200 slides), making its use in routine
histological prac-tice feasible. The APES concentrate is a toxic chemical that is moisture
sensitive and requires storage at 0-5 C; it is not carcinogenic but is an irritant and can cause …
immunostaining of pre-fixed frozen sections. It also provides a useful method of stabilising
histological and cytological prepara-tions on glass slides for other purposes. It is a
sufficiently simple, low cost technique (@ 50 pence for 200 slides), making its use in routine
histological prac-tice feasible. The APES concentrate is a toxic chemical that is moisture
sensitive and requires storage at 0-5 C; it is not carcinogenic but is an irritant and can cause …
Discussion
An alcoholic solution of APES fulfils the requirements for an adhesive for the immunostaining of pre-fixed frozen sections. It also provides a useful method of stabilising histological and cytological prepara-tions on glass slides for other purposes. It is a sufficiently simple, low cost technique (@ 50 pence for 200 slides), making its use in routine histological prac-tice feasible.
The APES concentrate is a toxic chemical that is moisture sensitive and requires storage at 0-5 C; it is not carcinogenic but is an irritant and can cause burns. Normal laboratory precautions, including the wearing of gloves and the use of a fume cupboard, should be taken when handling the concentrate solution. The method offers considerable benefit in saving laboratory time as it becomes possible to use rou-tinely available spare sections for techniques suchas immunocytochemistry andin situ hybridisation or 1256
ncbi.nlm.nih.gov
