Recombined CC chemokine ligand 2 into B16 cells induces production of Th2-dominanted cytokines and inhibits melanoma metastasis
K Hu, J Xiong, K Ji, H Sun, J Wang, H Liu - Immunology Letters, 2007 - Elsevier
This study is aimed to verify whether CCL2 can induce Th2 polarization in vivo and
subsequently inhibit tumor metastasis. B16 cells (a murine melanoma cell line) highly
expressing CCL2 (CCL2-B16 cells) were obtained by transfection with recombinant plasmid
CCL2-pcDNA3. Primary thymocytes were co-cultured with CCL2-B16 cells and STAT-6-
mediated Th2 polarization was noticed after co-culture. Caudal vein injection of CCL2-B16
cells effectively inhibited pulmonary metastasis in C57BL/6 mice, but not in nude mice …
subsequently inhibit tumor metastasis. B16 cells (a murine melanoma cell line) highly
expressing CCL2 (CCL2-B16 cells) were obtained by transfection with recombinant plasmid
CCL2-pcDNA3. Primary thymocytes were co-cultured with CCL2-B16 cells and STAT-6-
mediated Th2 polarization was noticed after co-culture. Caudal vein injection of CCL2-B16
cells effectively inhibited pulmonary metastasis in C57BL/6 mice, but not in nude mice …
Recombined CC chemokine ligand 2 into B16 cells induces production of Th2-dominant [correction of dominanted] cytokines and inhibits melanoma metastasis.
K Hu, J Xiong, K Ji, H Sun, J Wang, H Liu - Immunology Letters, 2007 - europepmc.org
This study is aimed to verify whether CCL2 can induce Th2 polarization in vivo and
subsequently inhibit tumor metastasis. B16 cells (a murine melanoma cell line) highly
expressing CCL2 (CCL2-B16 cells) were obtained by transfection with recombinant plasmid
CCL2-pcDNA3. Primary thymocytes were co-cultured with CCL2-B16 cells and STAT-6-
mediated Th2 polarization was noticed after co-culture. Caudal vein injection of CCL2-B16
cells effectively inhibited pulmonary metastasis in C57BL/6 mice, but not in nude mice …
subsequently inhibit tumor metastasis. B16 cells (a murine melanoma cell line) highly
expressing CCL2 (CCL2-B16 cells) were obtained by transfection with recombinant plasmid
CCL2-pcDNA3. Primary thymocytes were co-cultured with CCL2-B16 cells and STAT-6-
mediated Th2 polarization was noticed after co-culture. Caudal vein injection of CCL2-B16
cells effectively inhibited pulmonary metastasis in C57BL/6 mice, but not in nude mice …