Measurement of proton leak and electron leak in isolated mitochondria
C Affourtit, CL Quinlan, MD Brand - Mitochondrial bioenergetics: Methods …, 2012 - Springer
C Affourtit, CL Quinlan, MD Brand
Mitochondrial bioenergetics: Methods and protocols, 2012•SpringerOxidative phosphorylation is an important energy-conserving mechanism coupling
mitochondrial electron transfer to ATP synthesis. Coupling between respiration and
phosphorylation is not fully efficient due to proton and electron leaks. In this chapter,
methods are presented to measure proton and electron leak activities in isolated
mitochondria. The relative strength of a modular kinetic approach to probe oxidative
phosphorylation is emphasised.
mitochondrial electron transfer to ATP synthesis. Coupling between respiration and
phosphorylation is not fully efficient due to proton and electron leaks. In this chapter,
methods are presented to measure proton and electron leak activities in isolated
mitochondria. The relative strength of a modular kinetic approach to probe oxidative
phosphorylation is emphasised.
Abstract
Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.
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