Serial determination of glomerular filtration rate in conscious mice using FITC-inulin clearance

Z Qi, I Whitt, A Mehta, J Jin, M Zhao… - American Journal …, 2004 - journals.physiology.org
Z Qi, I Whitt, A Mehta, J Jin, M Zhao, RC Harris, AB Fogo, MD Breyer
American Journal of Physiology-Renal Physiology, 2004journals.physiology.org
Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious
mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured
GFR using clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on
a two-compartment model, estimated GFR was 236.69±16.55 and 140.20±22.27 μl/min in
male and female C57BL/6J mice, respectively. Total or ⅚ nephrectomy reduced inulin
clearance to 0 or 32.80±9.32 μl/min, respectively. Conversely, diabetes mellitus induced by …
Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured GFR using clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on a two-compartment model, estimated GFR was 236.69 ± 16.55 and 140.20 ± 22.27 μl/min in male and female C57BL/6J mice, respectively. Total or ⅚ nephrectomy reduced inulin clearance to 0 or 32.80 ± 9.32 μl/min, respectively. Conversely, diabetes mellitus induced by streptozotocin was associated with increased GFR. The other approach measured urinary inulin clearance using intraperitoneal microosmotic pumps to deliver FITC-inulin and metabolic cages to collect timed urine samples. This approach yielded similar GFR values of 211.11 ± 26.56 and 157.36 ± 20.02 μl/min in male and female mice, respectively. These studies demonstrate the feasibility of repeated nonisotopic measurement of inulin clearance in conscious mice.
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