[HTML][HTML] Hypoxia-inducible factor 1 alpha and vascular endothelial growth factor overexpression in ischemic colitis
T Okuda, T Azuma, M Ohtani, R Masaki… - World Journal of …, 2005 - ncbi.nlm.nih.gov
T Okuda, T Azuma, M Ohtani, R Masaki, Y Ito, Y Yamazaki, S Ito, M Kuriyama
World Journal of Gastroenterology: WJG, 2005•ncbi.nlm.nih.govAIM: To examine the etiology and pathophysiology in human ischemic colitis from the
viewpoint of ischemic factors such as hypoxia-inducible factor 1 alpha (HIF-1 alpha and
vascular endothelial growth factor (VEGF). METHODS: Thirteen patients with ischemic colitis
and 21 normal controls underwent colonoscopy. The follow-up colonoscopy was performed
in 8 patients at 7 to 10 d after the occurrence of ischemic colitis. Biopsy samples were
subjected to real-time RT-PCR and immunohistochemistry to detect the expression of HIF-1 …
viewpoint of ischemic factors such as hypoxia-inducible factor 1 alpha (HIF-1 alpha and
vascular endothelial growth factor (VEGF). METHODS: Thirteen patients with ischemic colitis
and 21 normal controls underwent colonoscopy. The follow-up colonoscopy was performed
in 8 patients at 7 to 10 d after the occurrence of ischemic colitis. Biopsy samples were
subjected to real-time RT-PCR and immunohistochemistry to detect the expression of HIF-1 …
Abstract
AIM: To examine the etiology and pathophysiology in human ischemic colitis from the viewpoint of ischemic factors such as hypoxia-inducible factor 1 alpha (HIF-1 alpha and vascular endothelial growth factor (VEGF).
METHODS: Thirteen patients with ischemic colitis and 21 normal controls underwent colonoscopy. The follow-up colonoscopy was performed in 8 patients at 7 to 10 d after the occurrence of ischemic colitis. Biopsy samples were subjected to real-time RT-PCR and immunohistochemistry to detect the expression of HIF-1 alpha and VEGF.
RESULTS: HIF-1 alpha and VEGF expression were found in the normal colon tissues by RT-PCR and immunohistochemistry. HIF-1 alpha and VEGF were overexpressed in the lesions of ischemic colitis. Overexpressed HIF-1 alpha and VEGF RNA quickly decreased to the normal level in the scar regions at 7 to 10 d after the occurrence of ischemic colitis.
CONCLUSION: Constant expression of HIF-1 alpha and VEGF in normal human colon tissue suggested that HIF-1 alpha and VEGF play an important role in maintaining tissue integrity. We confirmed the ischemic crisis in ischemic colitis at the molecular level, demonstrating overexpression of HIF-1 alpha and VEGF in ischemic lesions. These ischemic factors may play an important role in the pathophysiology of ischemic colitis.
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