Investigation of macrophage polarization using bone marrow derived macrophages

W Ying, PS Cheruku, FW Bazer, SH Safe… - JoVE (Journal of …, 2013 - jove.com
The article describes a readily easy adaptive in vitro model to investigate macrophage
polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from
the bone marrow are directed into monocytic differentiation, followed by M1 or M2
stimulation. The activation status can be tracked by changes in cell surface antigens, gene
expression and cell signaling pathways.