AGE‐DEPENDENT ALTERATIONS IN VASCULAR SMOOTH MUSCLE CELL RESPONSIVENESS TO PLATELET‐DERIVED GROWTH FACTOR IN GENETIC …
J Saltis, A Agrotis, A Bobik - Clinical and experimental …, 1993 - Wiley Online Library
J Saltis, A Agrotis, A Bobik
Clinical and experimental pharmacology and physiology, 1993•Wiley Online LibraryThis study examined and compared the growth characteristics of vascular smooth muscle
cells (VSMC) isolated from 4 and 12 week old spontaneously hypertensive rats (SHR) and
Wistar‐Kyoto (WKY) rats following stimulation with platelet‐derived growth factor‐BB (PDGF‐
BB). 2. Vascular smooth muscle cells from 4 week old SHR proliferated at a slower rate than
VSMC isolated from 12 week old SHR (1.08±0.06/day vs 1.89±0.13/day respectively, P<
0.05). In contrast, there was no difference in the proliferation of VSMC from 4 and 12 week …
cells (VSMC) isolated from 4 and 12 week old spontaneously hypertensive rats (SHR) and
Wistar‐Kyoto (WKY) rats following stimulation with platelet‐derived growth factor‐BB (PDGF‐
BB). 2. Vascular smooth muscle cells from 4 week old SHR proliferated at a slower rate than
VSMC isolated from 12 week old SHR (1.08±0.06/day vs 1.89±0.13/day respectively, P<
0.05). In contrast, there was no difference in the proliferation of VSMC from 4 and 12 week …
Summary
1. This study examined and compared the growth characteristics of vascular smooth muscle cells (VSMC) isolated from 4 and 12 week old spontaneously hypertensive rats (SHR) and Wistar‐Kyoto (WKY) rats following stimulation with platelet‐derived growth factor‐BB (PDGF‐BB).
2. Vascular smooth muscle cells from 4 week old SHR proliferated at a slower rate than VSMC isolated from 12 week old SHR (1.08±0.06/day vs 1.89±0.13/day respectively, P < 0.05). In contrast, there was no difference in the proliferation of VSMC from 4 and 12 week WKY rats (0.62±0.06/day vs 0.82±0.13/day, respectively, P>0.05).
3. The cell density at which VSMC from 4 and 12 week old SHR become refractory to the mitogenic effects of PDGF‐BB was similar and approximately two‐fold greater than VSMC from age‐matched WKY rats.
4. This study concludes that there is an age‐dependent, differential and specific upregulation of growth rate mechanisms in VSMC from SHR which enhance proliferation in response to PDGF‐BB.
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