Oligodendrocytes up-regulate the invasive activity of glioblastoma cells via the angiopoietin-2 signaling pathway

T Kawashima, M Yashiro, H Kasashima… - Anticancer …, 2019 - ar.iiarjournals.org
T Kawashima, M Yashiro, H Kasashima, Y Terakawa, T Uda, K Nakajo, R Umaba, Y Tanoue…
Anticancer research, 2019ar.iiarjournals.org
Background/Aim: Glioblastoma (GBM) is one of the most lethal solid cancers due to its highly
invasive nature. The malignant potential of GBM cells might be partially regulated by
surrounding normal cells, such as oligodendrocytes or fibroblasts. The aim of this study was
to examine the interaction between stromal cells and GBM cells. Materials and Methods:
Two GBM cell lines were used. The effect of stromal cells, oligodendrocytes or fibroblasts, on
the invasive ability of GBM cells was examined by wound-healing assay and invasion assay …
Background/Aim
Glioblastoma (GBM) is one of the most lethal solid cancers due to its highly invasive nature. The malignant potential of GBM cells might be partially regulated by surrounding normal cells, such as oligodendrocytes or fibroblasts. The aim of this study was to examine the interaction between stromal cells and GBM cells.
Materials and Methods
Two GBM cell lines were used. The effect of stromal cells, oligodendrocytes or fibroblasts, on the invasive ability of GBM cells was examined by wound-healing assay and invasion assay.
Results
Oligodendrocytes, in contrast to fibroblasts, significantly increased the migration and invasive ability of GBM cells. Angiopoietin-2 levels were high in the conditioned medium obtained from oligodendrocytes. Angiopoietin-2 significantly increased the motility of GBM, and the motility-stimulating activity of the oligodendrocytes-derived conditioned medium was significantly decreased by anti-angiopoietin-2-neutralizing antibody.
Conclusion
Glioma stromal cells, oligodendrocytes, might up-regulate the invasiveness of GBM cells via angiopoietin-2 signaling.
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