To evaluate the efficacy and toxicity of dendritic cell (DC) based therapy for intracerebral
gliomas, we utilized a cell line derived from an astrocytoma that arose spontaneously in a
VM/Dk mouse. This astrocytoma mirrors human gliomas phenotypically, morphologically
and secretes transforming growth factor (TGF)-βs, immunosuppressive cytokines secreted
by human gliomas. Systemic vaccination of mice with DCs pulsed with tumor homogenate
followed by intracranial tumor challenge produced a> 160% increase in median survival (p …