[HTML][HTML] pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains
HS Duffy, PL Sorgen, ME Girvin, P O'Donnell… - Journal of Biological …, 2002 - ASBMB
pH-induced closure of connexin43 (Cx43) channels involves interaction of the Cx43
carboxyl-terminal (Cx43CT) with a separate" receptor" domain. The receptor location and
structure and whether the interaction is directly intramolecular are unknown. Here we show
resonant mirror technology, enzyme-linked sorbent assays, and nuclear magnetic
resonance (NMR) experiments demonstrating pH-dependent binding of Cx43CT to region
119–144 of Cx43 (Cx43L2), which we propose is the receptor. NMR showed that …
carboxyl-terminal (Cx43CT) with a separate" receptor" domain. The receptor location and
structure and whether the interaction is directly intramolecular are unknown. Here we show
resonant mirror technology, enzyme-linked sorbent assays, and nuclear magnetic
resonance (NMR) experiments demonstrating pH-dependent binding of Cx43CT to region
119–144 of Cx43 (Cx43L2), which we propose is the receptor. NMR showed that …
