[HTML][HTML] Cytoplasmic Dynein Intermediate Chain Phosphorylation Regulates Binding to Dynactin* 210
PS Vaughan, JD Leszyk, KT Vaughan - Journal of Biological Chemistry, 2001 - ASBMB
Previously, we identified dynactin as a cargo receptor or adaptor for cytoplasmic dynein,
mediated by an interaction between the dynein intermediate chain and p150 Glued. To test
phosphorylation as a potential regulatory mechanism for this interaction, we analyzed
cytoplasmic dynein by two-dimensional gel analysis and detected two intermediate chain
variants, one of which was eliminated by phosphatase treatment. Overlay assays
demonstrated that p150 Glued bound dephosphorylated but not phosphorylated …
mediated by an interaction between the dynein intermediate chain and p150 Glued. To test
phosphorylation as a potential regulatory mechanism for this interaction, we analyzed
cytoplasmic dynein by two-dimensional gel analysis and detected two intermediate chain
variants, one of which was eliminated by phosphatase treatment. Overlay assays
demonstrated that p150 Glued bound dephosphorylated but not phosphorylated …
